Learn how immunoelectrophoresis identifies proteins based on their combined electrophoretic and immunological properties. This method is useful to monitor antigen and antigen-antibody purity and to identify a single antigen in a mixture of antigens. In this experiment, serum proteins are separated by agarose gel electrophoresis and the point of equivalence is observed by the antigen-antibody complex formation.
‚Ä¢ Explore electrophoresis and antigen-antibody interactions
‚Ä¢ Learn to identify proteins based on their electrophoretic and immunological properties
‚Ä¢ Discuss the research and clinical applications of immunoelectrophoresis
‚Ä¢ Perform electrophoresis on antigens, introduce antibodies, then analyze lines of precipitation
Instructions, proteins, antibodies; reagents, agarose, buffer, transfer pipets, well cutters, tubes, filter paper, 10mL pipettes, & 60mm petri plates.
All You Need:
Horizontal electrophoresis apparatus, power supply, automatic micropipettes with tips, microscope slides (1" x 3"), distilled water, 400-600 mL beaker, 1000 mL graduated cylinder, microwave, humidifying chamber, paper towels, plastic wrap or foil, & 37ÀöC incubation oven (optional).