Introduce your students to the principles of antigen-antibody interactions by using the Ouchterlony procedure. Antibodies and antigens form complexes that precipitate, making it possible to assay antibody-antigen systems. The binding interaction results in the formation of a white precipitate after diffusion in agarose.
‚Ä¢ Explore antigen-antibody interactions using a classic immunological technique
‚Ä¢ Set up Ouchterlony double diffusion plates and observe different precipitation reactions
‚Ä¢ Understand the biology behind equivalence zones
‚Ä¢ Recognize the patterns created by different reactions (identity, partial identity, non-identity)
Instructions, animal serum antigens & antibodies, practice gel loading solution, agarose, powdered buffer, transfer pipets, petri plates, well cutters, microtest tubes.
All You Need:
Automatic micropipettes with tips, 5 or 10 ml pipets, 55¬∫C water bath, measuring spatulas or toothpicks, microwave or hot plate, distilled water, incubation oven (optional).