The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (generating 5 fragments with distinct sizes and 2 fragments that are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analyzed by agarose gel electrophoresis.
‚Ä¢ Introduce restriction enzymes and their specificity
‚Ä¢ Prepare control and¬†EcoRI reactions of lambda phage DNA
‚Ä¢ Use electrophoresis to visualize and size uncut and digested samples
‚Ä¢ Explore restriction enzyme mapping
Instructions, Lambda phage DNA, Concentrated Restriction Enzyme Reaction Buffer, EDVOTEK¬Æ Enzyme Grade Water,¬†Eco¬†RI Dryzymes‚Ñ¢ endonuclease, DNA Standard Marker, Reconstitution Buffers, UltraSpec-Agarose‚Ñ¢, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, FlashBlue‚Ñ¢ DNA Stain, InstaStain‚Ñ¢ Blue Cards, Disposable Pipets, Microtest Tubes, & Semi-log Graph Paper Template.
All You Need:
DNA Electrophoresis,¬†Micropipettes: 5-50 ¬µl,¬†Water Bath,¬†White Light Box, & Microwave or Hot Plate.