The DNA from bacteriophage lambda is a well-characterized linear molecule containing six recognition sites for Eco RI (generating 5 fragments with distinct sizes and 2 fragments that are very close in size). In this experiment, Lambda DNA is digested by the Eco RI endonuclease. The digestion products are analyzed by agarose gel electrophoresis.
• Introduce restriction enzymes and their specificity
• Prepare control and EcoRI reactions of lambda phage DNA
• Use electrophoresis to visualize and size uncut and digested samples
• Explore restriction enzyme mapping
Kit Includes:
Instructions, Lambda phage DNA, Concentrated Restriction Enzyme Reaction Buffer, EDVOTEK® Enzyme Grade Water, Eco RI Dryzymes™ endonuclease, DNA Standard Marker, Reconstitution Buffers, UltraSpec-Agarose™, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, FlashBlue™ DNA Stain, InstaStain™ Blue Cards, Disposable Pipets, Microtest Tubes, & Semi-log Graph Paper Template.
All You Need:
DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, White Light Box, & Microwave or Hot Plate.
