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Identification and Characterization of Bacteria (625)


Gram staining is a quick, effective, and medically relevant identification test that has beome one of the most essential tools in bacterial classification. In this experiment, students will use this staining technique to examine the size, shape, arrangement, and gram status (+/-) of Escherichia coli, Bacillus subtilis, and Micrococcus luteus.

• Understand gram staining and its medical importance
• Explore bacteria diversity and classification
• Practice slide preparation, cell staining, and microscope observation
• Identify common bacteria based on size, shape, arrangement, and gram status

Kit Includes:

Instructions, BactoBeads‚Ñ¢, bottle of ReadyPour‚Ñ¢ Luria Broth Agar, large petri plates, 10 mL sterile pipet, inoculating loops, toothpicks, transfer pipets, microscope slides, Gram's Crystal Violet, Gram's Iodine, Gram's Safranin, sterile water, microcentrifuge tubes

All You Need:

Microscopes, microscope cover slips and oil (if required), microwave, pipet pump, incubator (recommended), timers, slide holders or clothespins, bunsen burner or alcohol lamp, lab marker, beakers, small staining tray, squeeze bottles (recommended), paper towels or blotting paper, 95% ethanol, distilled water, gloves