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Using CRISPR To Treat Cystic Fibrosis (580)


In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.

Kit Includes:

Instructions, Ready-to-Load QuickStrip‚Ñ¢ DNA Samples, UltraSpec-Agarose‚Ñ¢, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue‚Ñ¢ DNA Stain.

All You Need:

DNA Electrophoresis, Micropipettes: 5-50 µl (Optional), White Light Box, & Microwave or Hot Plate.