In this experiment, students will simulate the use of CRISPR-Cas9 to target a genetic mutation found in a patient suffering from Cystic Fibrosis. Students will develop an understanding of guide RNA (gRNA) design, and use agarose gel electrophoresis to examine pre-prepared DNA samples after CRISPR treatment.
Instructions, Ready-to-Load QuickStrip‚Ñ¢ DNA Samples, UltraSpec-Agarose‚Ñ¢, Electrophoresis Buffer (50X), 10X Gel Loading Solution, FlashBlue‚Ñ¢ DNA Stain.
All You Need:
DNA Electrophoresis, Micropipettes: 5-50 ¬µl (Optional), White Light Box, & Microwave or Hot Plate.